Journal: BMC Cancer

Article Title: PLCD1 expression for early detection and prognosis in High-Grade serous ovarian cancer

doi: 10.1186/s12885-025-15002-1

Figure Lengend Snippet: PLCD1 overexpression was observed in HGSOC tissues. (A) Representative IHC images of PLCD1 expression in normal, borderline, and HGSOC tissues (magnification ×40; scale bar, 50 μm). The mean IHC scores are presented as bar graphs for normal ( n = 68), borderline ( n = 44), and HGSOC ( n = 101) tissues. Statistical significance was determined by unpaired t-test (* P < 0.05, **** P < 0.0001). (B) Kaplan-Meier survival curves for 101 patients stratified by PLCD1 expression. The curves show differences in OS and DFS between low and high PLCD1 expression groups. Statistical analysis was performed using the log-rank test

Article Snippet: The PLCD1 lentiviral ORF cDNA plasmid with c-GFP tag (pLV-PLCD1-GFPSpark, HG18554-ACGLN) and Control Plasmid (pLV-C-GFPSpark, LVCV-35) were purchased from Sino Biological.

Techniques: Over Expression, Expressing

Journal: BMC Cancer

Article Title: PLCD1 expression for early detection and prognosis in High-Grade serous ovarian cancer

doi: 10.1186/s12885-025-15002-1

Figure Lengend Snippet: PLCD1 knockdown enhanced HGSOC cell proliferation in vitro. (A) PLCD1 expression in HGSOC cell lines were measured by Western blotting. α-actinin was used as a loading control. (B) OVCA429 cells were treated with varying concentrations of PLCD1-siRNA (50–200 nM) for 48 h. PLCD1 expression was assessed by Western blotting, with untreated cells (lane 1) and siControl-treated cells (lane 2) included. β-actin was used as a loading control. (C) Colony formation following PLCD1 knockdown in OVCA429 cells. Representative crystal violet-stained images are shown. (D) Quantification of colony formation by absorbance at 595 nm (left) and fold change analysis (right). Data are shown as mean ± SD. Statistical analysis by Mann–Whitney U test ( P < 0.05). (E) Invasion assay in OVCA429 cells following PLCD1 knockdown. Representative images (left) and quantification of invaded cells (right). Data are shown as mean ± SD (ns: not significant; Mann–Whitney U test)

Article Snippet: The PLCD1 lentiviral ORF cDNA plasmid with c-GFP tag (pLV-PLCD1-GFPSpark, HG18554-ACGLN) and Control Plasmid (pLV-C-GFPSpark, LVCV-35) were purchased from Sino Biological.

Techniques: Knockdown, In Vitro, Expressing, Western Blot, Control, Staining, MANN-WHITNEY, Invasion Assay

Journal: BMC Cancer

Article Title: PLCD1 expression for early detection and prognosis in High-Grade serous ovarian cancer

doi: 10.1186/s12885-025-15002-1

Figure Lengend Snippet: PLCD1 overexpression suppressed HGSOC cell proliferation in vitro. (A) OVCAR3 cells were transduced with a GFP-PLCD1 vector or empty control and PLCD1 expression was assessed by Western blotting. PLCD1 and α-actinin lanes were obtained from independent blots performed on different days under identical experimental conditions. α-actinin served as a common loading control across blots. (B) Representative images of spheroids formed from PLCD1-overexpressing and control OVCAR3 cells (scale bar, 200 μm). Spheroids were cultured for 9 days. (C) Invasion assay of OVCAR3 cells transduced with PLCD1 or empty vector. Representative images (left) and quantification of invaded cells (right). Data are shown as mean ± SD (ns: not significant; Mann–Whitney U test)

Article Snippet: The PLCD1 lentiviral ORF cDNA plasmid with c-GFP tag (pLV-PLCD1-GFPSpark, HG18554-ACGLN) and Control Plasmid (pLV-C-GFPSpark, LVCV-35) were purchased from Sino Biological.

Techniques: Over Expression, In Vitro, Transduction, Plasmid Preparation, Control, Expressing, Western Blot, Cell Culture, Invasion Assay, MANN-WHITNEY

Journal: BMC Cancer

Article Title: PLCD1 expression for early detection and prognosis in High-Grade serous ovarian cancer

doi: 10.1186/s12885-025-15002-1

Figure Lengend Snippet: PLCD1 overexpression inhibited tumor growth in vivo. (A) OVCAR3 cells transduced with either PLCD1 or empty vector were injected subcutaneously into nude mice. Tumor volume was monitored for 35 days. (B) Representative images of tumors on day 35 in the PLCD1 ( n = 11) and Empty ( n = 4) groups (scale bar, 5 mm). (C) Final tumor volumes (left) and tumor weights (right) are shown as bar graphs (mean ± SD). Statistical significance was determined using the Mann–Whitney U test ( P < 0.05; ns: not significant)

Article Snippet: The PLCD1 lentiviral ORF cDNA plasmid with c-GFP tag (pLV-PLCD1-GFPSpark, HG18554-ACGLN) and Control Plasmid (pLV-C-GFPSpark, LVCV-35) were purchased from Sino Biological.

Techniques: Over Expression, In Vivo, Transduction, Plasmid Preparation, Injection, MANN-WHITNEY